The methods that are frequently used in my lab are those presented here. There are many other protocols in the published literature and other researchers can give you tips on methods that they have developed but have not put into publications. Never hesitate to ask anyone who is working with Pythium what they have found that works!

Learning Objectives

By the end of this module, you should be able to:

  • Identify protocols for isolating Pythium from various sources
  • Explain tricks for getting Pythium in pure culture
  • Explain some protocols for storing isolates of Pythium
  • Describe a protocol for inducing Pythium to form zoospores
Isolating from Plant Tissue
Isolating from Soil by Direct Plating
Isolating from Water by Baiting
Cleaning and Storing Isolates
Active Cultures on Agar
Part 1: Media for Pythium Culture
Part 2: Media for Pythium Culture