Timothy W. McNellis, Ph.D.

Timothy W. McNellis, Ph.D.

  • Associate Professor
318 Buckhout Lab (office)
307 Buckhout Lab (lab)

University Park, PA 16802

Areas of Expertise

  • Fire blight resistance in apple trees
  • Biologically-based approaches to fire blight disease control
  • Development of citrus greening-resistant citrus varieties
  • Molecular genetics of Erwinia amylovora pathogenicity
  • Development of tomatoes resistant to bacterial canker disease
  • Understanding rootstock-scion interactions in grafted apple trees

Education

  • B.A., Biology, Johns Hopkins University, 1989
  • Ph.D., Biology, Yale University, 1995

Areas of Interest

Molecular basis of plant responses to phytopathogenic bacteria
Molecular mechanisms of bacterial pathogenesis in plants
Fire blight disease of apples

Program Interests

My research group studies the interaction of plants with bacterial pathogens at the molecular level.  The overall goal of our research is to improve our understanding of how plants resist bacterial pathogens and how bacterial pathogens cause disease in plants.  We primarily study the disease of apples and pears called fire blight, which is caused by the bacterium Erwinia amylovora.  Other current research topics include citrus greening disease and bacterial canker of tomatoes.

Recent Publications

Klee, S.M., Mostafa, I., Chen, S., Dufresne, C., Lehman, B.L., Sinn, J.P., Peter, K.A., and McNellis, T.W. (2018) An Erwinia amylovora yjeK mutant exhibits reduced virulence, increased chemical sensitivity and numerous environmentally dependent proteomic alterations.  Molecular Plant Pathology 19:1667-1678

Ramos, L. S., Sinn, J. P., Lehman, B. L., Pfeufer, E. E., Peter, K. A., and McNellis, T. W. (2015).  Erwinia amylovora pyrC mutant is virulent despite pyrimidine auxotrophy.  Letters in Applied Microbiology 60:572-279, DOI:10.1111/lam.12417

Kaja, E., Szczesniak, M. W., Jensen, P. J., Axtell, M. J., McNellis, T. W., and Makalowska, I. (2015)  Identification of apple miRNAs and their potential role in fire blight resistance.  Tree Genetics & Genomes 11:812, DOI 10.1007/s11295-014-0812-3

Ramos, L. S., Lehman, B. L., Peter, K. A., and McNellis, T. W. (2014)  Mutation of the Erwinia amylovora argD gene causes arginine auxotrophy, nonpathogenicity in apples, and reduced virulence in pears.  Applied and Environmental Microbiology 80:6739-6749

Jensen, P. J., Fazio, G., Altman, N., Praul, C., and McNellis, T. W. (2014)  Mapping in an apple (Malus x domestica) F1 segregating population based on physical clustering of differentially expressed genes.  BMC Genomics 15:261

Ramos, L. S., Lehman, B. L., Sinn, J. P., Pfeufer, E. E., Halbrendt, N. O., and McNellis, T. W. (2013) The fire blight pathogen Erwinia amylovora requires the rpoN gene for pathogenicity in apple.  Molecular Plant Pathology 14:838-843

Singh, D. K, Laremore, T. N., Smith, P. B., Maximova, S. N., and McNellis, T. W. (2012) Knockdown of FIBRILLIN4 gene expression in apple decreases plastoglobule plastoquinone content.  PLoS One 7:e47547

Jensen, P. J., Halbrendt, N., Fazio, G., Makalowska, I., Altman, N., Praul, C., Maximova, S. N., Ngugi, H. K., Crassweller, R. M., Travis, J. W., and McNellis, T. W. (2012).  Rootstock-regulated gene expression patterns associated with fire blight resistance in apple.  BMC Genomics 13:9

Singh, D. K., and McNellis, T. W. (2011).  Fibrillin protein function: the tip of the iceberg? Trends in Plant Science 16:432-441

Publications

Virulence genetics of an erwinia amylovora putative polysaccharide transporter family member
Journal of Bacteriology, Klee, Sara M., Sinn, Judith P., Christian, Elena, Holmes, Aleah C., Zhao, Kaixi, Lehman, Brian L., Peter, Kari A., Rosa, Cristina, McNellis, Timothy W., 2020

Flowering locus t chimeric protein induces floral precocity in edible citrus
Plant Biotechnology Journal, Sinn, Judith P., Held, Jeremy B., Vosburg, Chad, Klee, Sara M., Orbovic, Vladimir, Taylor, Earl L., Gottwald, Tim R., Stover, Ed, Moore, Gloria A., McNellis, Timothy W., 2020

Extragenic suppression of elongation factor P gene mutant phenotypes in erwinia amylovora
Journal of Bacteriology, Klee, Sara M., Sinn, Judith P., Holmes, Aleah C., Lehman, Brian L., Krawczyk, Teresa, Peter, Kari A., McNellis, Timothy W., 2019

Erwinia amylovora auxotrophic mutant exometabolomics and virulence on apples
Applied and Environmental Microbiology, Klee, Sara M., Sinn, Judith P., Finley, Melissa, Allman, Erik L., Smith, Philip B., Aimufua, Osaretin, Sitther, Viji, Lehman, Brian L., Krawczyk, Teresa, Peter, Kari A., McNellis, Timothy W., 2019

The apple fruitlet model system for fire blight disease, Klee, Sara M., Sinn, Judith P., McNellis, Timothy W., 2019

An Erwinia amylovora yjeK mutant exhibits reduced virulence, increased chemical sensitivity and numerous environmentally dependent proteomic alterations
Molecular Plant Pathology, Klee, Sara M., Mostafa, Islam, Chen, Sixue, Dufresne, Craig, Lehman, brian L., Sinn, Judith P., Peter, Kari A., McNellis, Timothy W., 2018

Identification of apple miRNAs and their potential role in fire blight resistance
Tree Genetics and Genomes, Kaja, Elżbieta, Szcześniak, Michał W., Jensen, Philip J., Axtell, Michael J., McNellis, Timothy, Makałowska, Izabela, 2015

Erwinia amylovora pyrC mutant causes fire blight despite pyrimidine auxotrophy
Letters in Applied Microbiology, Ramos, L. S., Sinn, J. P., Lehman, B. L., Pfeufer, E. E., Peter, K. A., McNellis, T. W., 2015

Mapping in an apple (Malus x domestica) F1 segregating population based on physical clustering of differentially expressed genes
BMC Genomics, Jensen, Philip J., Fazio, Gennaro, Altman, Naomi, Praul, Craig, McNellis, Timothy W., 2014

Mutation of the Erwinia amylovora argD gene causes arginine auxotrophy, nonpathogenicity in apples, and reduced virulence in pears
Applied and Environmental Microbiology, Ramos, Laura S., Lehman, Brian L., Peter, Kari A., McNellis, Timothy W., 2014

Knockdown of FIBRILLIN4 gene expression in apple decreases plastoglobule plastoquinone content
PLoS One, Singh, Dharmendra K., Laremore, Tatiana N., Smith, Philip B., Maximova, Siela N., McNellis, Timothy W., 2012

Rootstock-regulated gene expression patterns associated with fire blight resistance in apple
BMC Genomics, Jensen, Philip J., Halbrendt, Noemi, Fazio, Gennaro, Makalowska, Izabela, Altman, Naomi, Praul, Craig, Maximova, Siela N., Ngugi, Henry K., Crassweller, Robert M., Travis, James W., McNellis, Timothy W., 2012

Fibrillin protein function
Trends in Plant Science, Singh, Dharmendra K., McNellis, Timothy W., 2011

Rootstock-regulated gene expression profiling in apple trees reveals genes whose expression levels are associated with fire blight resistance, Jensen, P. J., McNellis, T. W., Altman, N., Maximova, S. N., Crassweller, R. M., Halbrendt, N., Travis, J. W., Praul, C. A., Makalowska, I., 2011