Marilyn J. Roossinck, Ph.D.

Marilyn J. Roossinck, Ph.D.

  • Professor, Plant Pathology and Environmental Microbiology, and Biology
  • Center for Infectious Disease Dynamics, Huck Institute of Life Sciences
Center for Infectious Disease Dynamics
W229A Millennium Science Complex
The Pennsylvania State University

University Park, PA 16802

Areas of Expertise

  • Adaptation to extreme environments with microbes

Education

  • Ph.D., University of Colorado School of Medicine

Areas of Interest:

Our focus in the Roossinck lab is on virus-plant and virus-fungus-plant interactions in virus evolution and ecology.  

We use Cucumber mosaic virus (CMV) as a model virus for experimental evolution studies, including generation and maintenance of quasispecies, rates of recombination, and polymerase fidelity.

We are interested in how plants use viruses in adaptation to extreme environments. Fungal viruses are involved in plants adapting to geothermal soils in Yellowstone National Park, and acute plant viruses confer drought and cold tolerance to plants.  We are studying the mechanisms of these interactions.

We are interested in the biodiversity of plant viruses.  We are studying viruses in wild plants in Costa Rica using next-gen sequence analysis of viral RNA from individual plants.  To date we have collected about 14,000 plants and have analyzed about half of them.

Recent Publications:

 

Roossinck, Marilyn J. Virus: An Illustrated Guide to 101 Incredible Microbes. Princeton, NJ: Princeton University Press, 2016.

Research papers:

Simmonds, P., M.J. Adams, M. Benko, M. Breitbart, J.R. Brister, E.B. Carstens, A.J. Davison, E. Delwart, A.E. Gorbalenya, B. Harrach, R. Hull, A.M.Q. King, E.V. Koonin, M. Kuprovic,  J.H.Kuhn, E.J. Lefkowitz, M.L. Nibert, R. Orton, M.J. Roossinck, S. Sabanadzovic, M.B. Sullivan, C.A. Suttle, R.B. Tesh, R.A. vanderVlugt, A.Varsani, F.M. Zerbina. 2017. Consensus: Virus taxonomy in the age of metagenomics. Nature Reviews Microbiology,  15: 161-168

Ali, Akhtar, and M.J. Roossinck. 2017.  Analysis of quasispeces variation in single and mixed infections. Virus Evolution, 3 (2) vex037

Thapa, V., G.G. Turner, S. Hafenstein, B.E. Overton, K.J. Vanderwolf, M.J. Roossinck. 2016. Using a novel partitivirus in Pseudogymnoascus destructans to understand the epidemiology of white-nose syndrome. PLOS Pathogens 12:e1006076.

Min, B.-E., T.S. Feldman, A. Ali, G. Wiley, V. Muthukumar,  B.A. Roe, M. J. Roossinck, U. Melcher, M. W. Palmer, R. S. Nelson. 2012. Cloning, ecological and epidemiological analysis of a novel tymovirus in Asclepias viridis from Oklahoma. Phytopathology 102:166-176. 

Stobbe, A.H.,  U. Melcher, M.W. Palmer, M. J. Roossinck, and G. Shen. 2012.  Co-divergence and host-switching in the evolution of tobamoviruses.  J. Gen. Virol. 93:408-418.

Feldman, T.S., M.R. Morsy, and M. J. Roossinck. 2012.  Are communities of microbial symbionts more diverse than communities of macrobial hosts? Fungal Biology 116: 465-477.

Thapa, V.  U. Melcher, G. B. Wiley, A. Doust, M. W. Palmer, K. Roewe, B. A. Roe, G. Shen, and M. J. Roossinck. 2012.  Detection of Members of the Secoviridae in the Tallgrass Prairie Preserve, Osage County, Oklahoma USA Virus Res. 167:34-42.

Xu, P. H. Wang, F. Coker, J-Y Ma, Y. Tang, ME Taylor and MJ Roossinck 2012.  Genetic loci controlling lethal cell death in tomato caused by viral satellite RNA infection.  Mol. Plant-Microbe Interact. 25:1034-1044

Pita, J. S., and M. J. Roossinck.  2013. Mapping viral functional domains for genetic diversity in plants. J. Virol. 87:790-797

Pita, J.S. and M.J. Roossinck. 2013. Fixation of emerging inter-viral recombinants in Cucumber mosaic virus populations is controlled by the replicase. J. Virol. 87:1264-1269

MacDiarmid, R., B. Rodoni, U. Melcher, F. Ochoa-Corona, and M.J. Roossinck. 2013. Biosecurity implications of new technology in plant virus research. PLoS Pathogens 9:8

Du, Z., A. Chen, W. Chen, Q. Liao, H. Zhang, Y. Bao, M.J. Roossinck, and J.P. Carr. 2014.  Nuclearcytoplasmic partitioning of Cucumber mosaic virus protein 2b determines the balance between its roles as a virulence determinant and an RNA-silencing suppressor. J. Virol. 88:5228-5241.

Peyambari, M., M.K. Habibi, K-B. Fotouhifar, A Dizadji, and M.J. Roossinck. 2014.  Molecular characterization of a novel putative partitivurs infecting Cytospora sacchari, a plant pathogenic fungus.  Plant Pathol. J. 30:151-158.

Reviews:

Ouedraogo, R.S. and M.J. Roossinck. 2017. Chapter 18, Molecular Evolution, in Cucumber mosaic virus, P. Palukaitis and F. García-Arenal, editors. in press.

Roossinck, M.J.  and F. García-Arenal.  Chapter 25. Evolution of Cucumber mosaic virus Satellite RNAs, in Cucumber mosaic virus, P. Palukaitis and F. García-Arenal, editors. in press.

 Roossinck, M.J. 2016. Environmental Virology:  How domestic viruses impact wild species. Curr. Op. Virol. 19:v-vi

 Kim, M. and M.J. Roossinck. 2016. Small Linear Satellite RNAs. in Viroids and Plant Viral Satellite RNAs, P. Palikaitis, ed. in press.

Roossinck, M.J. 2016. Deep sequencing for discovery and evolutionary analysis of plant viruses. Virus Research invited review doi.org/10.1016/j.virusres.2016.11.019

Stobbe, A., and M.J. Roossinck. 2016. Plant virus diversity and evolution. in Current Research Topics in Plant Virology, A. Wang, ed. pp. 197-215.

Roossinck, M.J. 2016. Evolution of persistent viruses in plants. in Virus Evolution. Weaver, Roossinck, Denison and Vignuzzi. pp. 263-272.

Roossinck, M.J. 2015. Move over bacteria!  Viruses make their mark as mutualistic symbionts. J. Virol. invited JEMS article, 89:1-3

 Roossinck, M.J. 2015.  Plants, viruses and the environment:  ecology and mutualism.  Virology 60th Anniversary edition 479-480:271-277.

Roossinck, M.J., D.P. Martin and P. Roumagnac. 2015.  Plant virus metagenomics: Applications for plant pathology. Phytopathology 105:716-727.

Roossinck M.J. 2015. A new look at plant viruses and their potential roles in crops.  Mol. Plant Pathol. 16:331-333 

Roossinck, M.J. and F. García-Arenal. 2015.  Ecosystem simplification, biodiversity loss and plant virus emergence. Curr. Op. Virology 10:56-62.

Roossinck, M.J. 2015.  Metagenomics of plant and fungal viruses reveals an abundance of persistent lifestyles. Frontiers in Microbiology 5:e787.

Stobbe, A.H. and M.J. Roossinck. 2015.  Plant Virus Metagenomics:  what we know and why we need to know more.  Frontiers in Plant Science 5:e150.

Safari, M. and M. J. Roossinck. 2014.  How does the genome structure and lifestyle of a virus affect its population variation?  Curr. Op Virology 9:39-44

Bao, X., and M. J. Roossinck. 2013.  A Life History View of Mutualistic Viral Symbioses: Quantity or Quality for Cooperation?  Curr. Op. Microbiol., 16:514-518.

Roossinck, M.J. 2013. Plant Virus Ecology. PLoS Pathogens 9(5): e1003304

Bao, X., and M. J. Roossinck. 2013. Multiplexed interactions:  viruses of endophytic fungi. Adv. in Virus Res. Adv. in Virus Res.  86:37-57.

Rodriguez, R.J., and M.J. Roossinck. 2012. Viruses, fungi and plants:  Cross-Kingdom Communication and Mutualism. in Biocommunication of Fungi, pp. 219-227

Roossinck, M. J. 2012. Persistent Plant Viruses:  Molecular Hitchhikers or Epigenetic Elements?  In Viruses:  Essential Agents of Life. pp 177-186.

Roossinck, M. J. 2012. Plant virus metagenomics:  Biodiversity and ecology.  Ann. Rev. Genetics 46:357-367.

Márquez, L. M. and M. J. Roossinck. 2012.  Do persistent viruses fit the trade-off hypothesis of virulence evolution?  Curr. Op. Virol. 2:556-560.

Roossinck, M. J. 2012.  Even viruses can be beneficial microbes.  Microbiol. Australia 33: 111-112

Publications

Manipulation of aphid behavior by a persistent plant virus
Journal of virology, Safari, Maliheh, Ferrari, Matthew J., Roossinck, Marilyn J., 2019

Impact of cultivated hosts on the recombination of cucumber mosaic virus
Journal of virology, Ouedraogo, Rimnoma S., Pita, Justin S., Somda, Irenée P., Traore, Oumar, Roossinck, Marilyn J., 2019

A 1,000-year-old RNA virus
Journal of virology, Peyambari, Mahtab, Warner, Sylvia, Stoler, Nicholas, Rainer, Drew, Roossinck, Marilyn J., 2019

Large-scale synonymous substitutions in cucumber mosaic virus RNA 3 facilitate amino acid mutations in the coat protein
Journal of virology, Mochizuki, Tomofumi, Ohara, Rie, Roossinck, Marilyn J., 2018

Coevolution of a persistent plant virus and its pepper hosts
Molecular Plant-Microbe Interactions, Safari, Maliheh, Roossinck, Marilyn J., 2018

Evolutionary and ecological links between plant and fungal viruses
New Phytologist, Roossinck, Marilyn J., 2018

ICTV virus taxonomy profile
Journal of General Virology, Vainio, Eeva J., Chiba, Sotaro, Ghabrial, Said A., Maiss, Edgar, Roossinck, Marilyn, Sabanadzovic, Sead, Suzuki, Nobuhiro, Xie, Jiatao, Nibert, Max, 2018

Symbiosis
Annual Review of Virology, Roossinck, Marilyn J., Bazán, Edelio R., 2017

Consensus statement
Nature Reviews Microbiology, Simmonds, Peter, Adams, Mike J., Benk, Mária, Breitbart, Mya, Brister, J. Rodney, Carstens, Eric B., Davison, Andrew J., Delwart, Eric, Gorbalenya, Alexander E., Harrach, Balázs, Hull, Roger, King, Andrew M.Q., Koonin, Eugene V., Krupovic, Mart, Kuhn, Jens H., Lefkowitz, Elliot J., Nibert, Max L., Orton, Richard, Roossinck, Marilyn J., Sabanadzovic, Sead, Sullivan, Matthew B., Suttle, Curtis A., Tesh, Robert B., Van Der Vlugt, René A., Varsani, Arvind, Zerbini, F. Murilo, 2017

A new look at plant viruses and their potential beneficial roles in crops
Molecular plant pathology, Roossinck, Marilyn J., 2015

Differential responses to virus challenge of laboratory and wild accessions of Australian species of Nicotiana, and comparative analysis of RDR1 gene sequences
PloS one, Wylie, Stephen J., Zhang, Chao, Long, Vicki, Roossinck, Marilyn J., Koh, Shu Hui, Jones, Michael G.K., Iqbal, Sadia, Li, Hua, 2015

Mutation and recombination frequencies reveal a biological contrast within strains of Cucumber Mosaic Virus
Journal of virology, Pita, Justin S., Morris, Viktoriya, Roossinck, Marilyn J., 2015

Move over, bacteria! viruses make their mark as mutualistic microbial symbionts
Journal of virology, Roossinck, Marilyn J., 2015

Ecosystem simplification, biodiversity loss and plant virus emergence
Current Opinion in Virology, Roossinck, Marilyn J., García-Arenal, Fernando, 2015

Determinants of taxonomic composition of plant viruses at the Nature Conservancy's Tallgrass Prairie Preserve, Oklahoma
Virus Evolution, Thapa, V, McGlinn, D, Melcher, U, Palmer, M, Roossinck, Marilyn, 2015

How does the genome structure and lifestyle of a virus affect its population variation?
Current Opinion in Virology, Safari, Maliheh, Roossinck, Marilyn J., 2014

Plant virus metagenomics
Frontiers in Plant Science, Stobbe, Anthony H., Roossinck, Marilyn J., 2014

Nuclear-cytoplasmic partitioning of cucumber mosaic virus protein 2b determines the balance between its roles as a virulence determinant and an rna-silencing suppressor
Journal of virology, Du, Zhiyou, Chen, Aizhong, Chen, Wenhu, Liao, Qiansheng, Zhang, Hengmu, Bao, Yiming, Roossinck, Marilyn J., Carr, John P., 2014

Molecular characterization of a novel putative partitivirus infecting Cytospora sacchari, a plant pathogenic fungus
Plant Pathology Journal, Peyambari, Mahtab, Habibi, Mina Koohi, Fotouhifar, Khalil Berdi, Dizadji, Akbar, Roossinck, Marilyn J., 2014

Biosecurity Implications of New Technology and Discovery in Plant Virus Research
PLoS pathogens, MacDiarmid, Robin, Rodoni, Brendan, Melcher, Ulrich, Ochoa-Corona, Francisco, Roossinck, Marilyn, 2013

Plant Virus Ecology
PLoS pathogens, Roossinck, Marilyn J., 2013

Multiplexed Interactions. Viruses of Endophytic Fungi.
Advances in virus research, Bao, Xiaodong, Roossinck, Marilyn J., 2013

Fixation of emerging interviral recombinants in Cucumber mosaic virus populations
Journal of virology, Pita, Justin S., Roossinck, Marilyn J., 2013

Mapping viral functional domains for genetic diversity in plants
Journal of virology, Pita, Justin S., Roossinck, Marilyn J., 2013

Do persistent RNA viruses fit the trade-off hypothesis of virulence evolution?
Current Opinion in Virology, Márquez, Luis M., Roossinck, Marilyn J., 2012

Genetic loci controlling lethal cell death in tomato caused by viral satellite RNA infection
Molecular Plant-Microbe Interactions, Xu, Ping, Wang, Hua, Coker, Frank, Ma, Jun Y.Ing, Tang, Yuhong, Taylor, Mark, Roossinck, Marilyn J., 2012

Detection of members of the Secoviridae in the Tallgrass Prairie Preserve, Osage County, Oklahoma, USA
Virus Research, Thapa, Vaskar, Melcher, Ulrich, Wiley, Graham B., Doust, Andrew, Palmer, Michael W., Roewe, Kimberly, Roe, Bruce A., Shen, Guoan, Roossinck, Marilyn J., Wang, Ye Margaret, Kamath, Nitin, 2012

Are communities of microbial symbionts more diverse than communities of macrobial hosts?
Fungal Biology, Feldman, Tracy S., Morsy, Mustafa R., Roossinck, Marilyn J., 2012

Molecular characterization, ecology, and epidemiology of a novel tymovirus in asclepias viridis from Oklahoma
PHYTOPATHOLOGY, Min, Byoung Eun, Feldman, Tracy S., Ali, Akhtar, Wiley, Graham, Muthukumar, Vijay, Roe, Bruce A., Roossinck, Marilyn J., Melcher, Ulrich, Palmer, Michael W., Nelson, Richard S., 2012

Co-divergence and host-switching in the evolution of tobamoviruses
Journal of General Virology, Stobbe, Anthony H., Melcher, Ulrich, Palmer, Michael W., Roossinck, Marilyn J., Shen, Guoan, 2012

Plant virus metagenomics
Annual review of genetics, Roossinck, Marilyn J., 2012

Bell pepper endornavirus
Journal of General Virology, Okada, Ryo, Kiyota, Eri, Sabanadzovic, Sead, Moriyama, Hiromitsu, Fukuhara, Toshiyuki, Saha, Prasenjit, Roossinck, Marilyn J., Severin, Ake, Valverde, Rodrigo A., 2011

The remarkable evolutionary history of endornaviruses
Journal of General Virology, Roossinck, Marilyn J., Sabanadzovic, Sead, Okada, Ryo, Valverde, Rodrigo A., 2011

Changes in population dynamics in mutualistic versus pathogenic viruses
Viruses, Roossinck, Marilyn J., 2011

The big unknown
Current Opinion in Virology, Roossinck, Marilyn J., 2011

Teasing apart a three-way symbiosis
Biochemical and Biophysical Research Communications, Morsy, Mustafa R., Oswald, Jennifer, He, Ji, Tang, Yuhong, Roossinck, Marilyn J., 2010

Genetic bottlenecks during systemic movement of Cucumber mosaic virus vary in different host plants
Virology, Ali, Akhtar, Roossinck, Marilyn J., 2010

Ecogenomics
Molecular ecology, Roossinck, Marilyn J., Saha, Prasenjit, Wiley, Graham B., Quan, Jiaxi, White, James D., Lai, Hongshing, Chavarría, Felipe, Shen, Guoan, Roe, Bruce A., 2010