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Penn State University College of Agricultural Sciences

Plant Pathology

Mushroom Spawn Lab

AgSci » Plant Pathology » Facilities » Mushroom Spawn Lab » Cultures/Spawn » How to Start a Culture

How to Start a Culture

Mushroom Cultures are started by Spore Print or by Tissue Culture.

Spore Print

Mushroom Spores are collected and germinated on a plate to create a new culture.

Spore Print - thumbnail

Spore Print - small

Example of Spore Print

Example of Spore Print - thumbnail

Example of Spore Print - small

Tissue Culture

Tissues are removed from the cap

Tissue Culture - thumbnail

Tissue Culture - small

Tissue Culture

Tissue grown out on a plate to create a clone of the original mushroom

Tissue Culture - thumbnail

Tissue Culture - small

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