PPEM 497A Genome Editing (2 credits)
Instructor: Yinong Yang, Ph.D.
Time: Fridays | 9:05-11:00 a.m.
Location: 201 Buckhout Laboratory
Instructor Contact Information:
Office: 405C Huck Life Sciences Building
Phone: (814) 867-0324
Genome editing has recently emerged as a powerful technology for precise genetic engineering of living organisms. This course will provide an in-depth overview of genome editing technologies mediated by programmable nucleases. Students are expected to gain a mechanistic understanding of various genome editing techniques as well as practical knowledge regarding the molecular design, genome manipulation, and genetic analysis. In addition, case studies will be presented to demonstrate the broad application of genome editing technology in basic biological research (e.g., functional genomics), medicine (e.g., gene therapy), and agriculture (e.g., precision crop breeding).
The class meets once a week (about two hours) for lecture and discussion. Lectures will consist primarily of presentations including the use of PowerPoint and blackboard. The class will also include case studies, virtual experiments, and student presentations. There will be frequent opportunities for discussion and class participation. There is no textbook available for this course. Reading materials (e.g., recent review articles and original papers), lecture slides, and other teaching resources will be posted on the ANGEL/CANVAS website.
|Requirement||Weight of Points|
|Class attendance and participation||10%|
|Case study presentation||40%|
Students have a responsibility to deal honestly with all members of the university community. Academic dishonesty will result in sanctions as stated in the University and Graduate School regulations.
|Aug 24||Introduction to genome engineering|
|Aug 31||HE, ZFN and TALEN editing technologies|
|Sept 7||CRISPR/Cas system: History and basic biology|
|Sept 14||Basic CRISPR/Cas9 technology for genome editing|
|Sept 21||Specificity/Off-target analysis and delivery systems|
|Sept 28||Advanced CRISPR/Cas9 technologies|
|Oct 5||Applications in basic research and biomedicine|
|Oct 12||Applications in agricultural biotechnology|
|Oct 19||Case study: Student presentation|
|Oct 26||Case study: Student presentation|
|Nov 2||Case study: Student presentation|
|Nov 9||Virtual experiment/discussion|
|Nov 16||Virtual experiment/discussion|
|Nov 23||Thanksgiving break|
|Nov 30||Challenge problems|
|Dec 7||Challenge problems|
|Dec 14||Final exams week|