PPEM 497A Genome Editing (2 credits)

Fall Semester 2016 PPEM 497A Genome Editing course syllabus.

Course Logistics

Instructor: Yinong Yang, Ph.D.

Time: Fridays | 9:05-11:00 a.m.

Location: 201 Buckhout Laboratory

Instructor Contact Information:
Office: 405C Huck Life Sciences Building
Phone: (814) 867-0324

Course Description

Genome editing has recently emerged as a powerful technology for precise genetic engineering of living organisms. This course will provide an in-depth overview of genome editing technologies mediated by programmable nucleases. Students are expected to gain a mechanistic understanding of various genome editing techniques as well as practical knowledge regarding the molecular design, genome manipulation, and genetic analysis. In addition, case studies will be presented to demonstrate the broad application of genome editing technology in basic biological research (e.g., functional genomics), medicine (e.g., gene therapy), and agriculture (e.g., precision crop breeding).

The class meets once a week (about two hours) for lecture and discussion. Lectures will consist primarily of presentations including the use of PowerPoint and blackboard. The class will also include case studies, virtual experiments, and student presentations. There will be frequent opportunities for discussion and class participation. There is no textbook available for this course. Reading materials (e.g., recent review articles and original papers), lecture slides, and other teaching resources will be posted on the ANGEL/CANVAS website.

Course Requirements

RequirementWeight of Points
Class attendance and participation 10%
Case study presentation 40%
Challenge problem 50%
TOTAL 100%

Academic Integrity

Students have a responsibility to deal honestly with all members of the university community. Academic dishonesty will result in sanctions as stated in the University and Graduate School regulations.

Course Calendar

Aug 24 Introduction to genome engineering
Aug 31 HE, ZFN and TALEN editing technologies
Sept 7 CRISPR/Cas system: History and basic biology
Sept 14 Basic CRISPR/Cas9 technology for genome editing
Sept 21 Specificity/Off-target analysis and delivery systems
Sept 28 Advanced CRISPR/Cas9 technologies
Oct 5 Applications in basic research and biomedicine
Oct 12 Applications in agricultural biotechnology
Oct 19 Case study: Student presentation
Oct 26 Case study: Student presentation
Nov 2 Case study: Student presentation
Nov 9 Virtual experiment/discussion
Nov 16 Virtual experiment/discussion
Nov 23 Thanksgiving break
Nov 30 Challenge problems
Dec 7 Challenge problems
Dec 14 Final exams week